WO2002101019A3 - Methods of culturing animal cells and polypeptide production in animal cells - Google Patents

Methods of culturing animal cells and polypeptide production in animal cells Download PDF

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Publication number
WO2002101019A3
WO2002101019A3 PCT/US2002/018687 US0218687W WO02101019A3 WO 2002101019 A3 WO2002101019 A3 WO 2002101019A3 US 0218687 W US0218687 W US 0218687W WO 02101019 A3 WO02101019 A3 WO 02101019A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
animal cells
polypeptides
cell culture
culturing
Prior art date
Application number
PCT/US2002/018687
Other languages
French (fr)
Other versions
WO2002101019A2 (en
Inventor
Neslihan Delacruz
Tina Etcheverry
Michael Ostland
Original Assignee
Genentech Inc
Neslihan Delacruz
Tina Etcheverry
Michael Ostland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc, Neslihan Delacruz, Tina Etcheverry, Michael Ostland filed Critical Genentech Inc
Priority to CA002447791A priority Critical patent/CA2447791A1/en
Priority to JP2003503770A priority patent/JP2004532642A/en
Priority to AU2002316230A priority patent/AU2002316230A1/en
Priority to EP02746513A priority patent/EP1404813A4/en
Publication of WO2002101019A2 publication Critical patent/WO2002101019A2/en
Publication of WO2002101019A3 publication Critical patent/WO2002101019A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Abstract

A method of improving expression of polypeptides in mammalian cell culture systems is provided. In particular, the invention is directed to methods of culturing mammalian cells under conditions such as, for example, high levels of glucose, where environmental and nutritional conditions are controlled and adjusted so as to improve process robustness and to promote superior expression of polypeptides having desired qualities, e.g., biological activity. The invention also relates to polypeptides, especially antibodies, produced by the methods, and to methods of using such antibodies, and to pharmaceutical compositions containing the polypeptides. The invention also relates to methods of culturing animal cells in a fed batch cell culture to maximize production capacity, viability of the cell culture, and overall enhancement of yield from such cultures.
PCT/US2002/018687 2001-06-13 2002-06-11 Methods of culturing animal cells and polypeptide production in animal cells WO2002101019A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002447791A CA2447791A1 (en) 2001-06-13 2002-06-11 Methods of culturing animal cells and polypeptide production in animal cells
JP2003503770A JP2004532642A (en) 2001-06-13 2002-06-11 Animal cell culture method and polypeptide production in animal cells
AU2002316230A AU2002316230A1 (en) 2001-06-13 2002-06-11 Methods of culturing animal cells and polypeptide production in animal cells
EP02746513A EP1404813A4 (en) 2001-06-13 2002-06-11 Methods of culturing animal cells and polypeptide production in animal cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29801301P 2001-06-13 2001-06-13
US60/298,013 2001-06-13

Publications (2)

Publication Number Publication Date
WO2002101019A2 WO2002101019A2 (en) 2002-12-19
WO2002101019A3 true WO2002101019A3 (en) 2003-04-10

Family

ID=23148629

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/018687 WO2002101019A2 (en) 2001-06-13 2002-06-11 Methods of culturing animal cells and polypeptide production in animal cells

Country Status (6)

Country Link
US (1) US20030087372A1 (en)
EP (1) EP1404813A4 (en)
JP (1) JP2004532642A (en)
AU (1) AU2002316230A1 (en)
CA (1) CA2447791A1 (en)
WO (1) WO2002101019A2 (en)

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Also Published As

Publication number Publication date
JP2004532642A (en) 2004-10-28
AU2002316230A1 (en) 2002-12-23
CA2447791A1 (en) 2002-12-19
EP1404813A4 (en) 2004-11-24
WO2002101019A2 (en) 2002-12-19
US20030087372A1 (en) 2003-05-08
EP1404813A2 (en) 2004-04-07

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